mouse cxcl2 elisa kit  (Proteintech)

Journal: Stem Cell Research & Therapy

Article Title: Enhanced therapeutic efficacy of repeated bone marrow–derived MSC administration in a murine model of pulmonary fibrosis

doi: 10.1186/s13287-025-04859-5

Figure Lengend Snippet: Single-Cell transcriptomic analysis reveals inflammatory immune landscape in pulmonary fibrosis. A tSNE visualization of 20 major cell types isolated from mouse lungs across four experimental groups: NS-7d, SiO₂-7d, NS-56d, and SiO₂-56d. B tSNE plot displaying single-cell transcriptional profiles from four experimental groups and the accompanying bar plot depicts dynamic changes in cell-type composition across time points, comparing silica- and saline-exposed mice. C , D Top 10 upregulated KEGG and GO MF terms in SiO2-7d. E Top 10 upregulated GO BP terms in SiO2-56d. F Visualization of immune scores across all single cells from NS-7d, SiO₂-7d, NS-56d, and SiO₂-56d groups projected onto the t-SNE plot. G Visualization of fibrosis scores across all single cells from NS-7d, SiO₂-7d, NS-56d, and SiO₂-56d groups projected onto the t-SNE plot. H Bubble plot showing the expression levels of Tnf, Il6 , and Cxcl2 in NS-7d, SiO₂-7d, NS-56d, and SiO₂-56d groups

Article Snippet: ELISA assays were performed using commercial Mouse TNF-α, IL-6, and CXCL2 ELISA kits (Proteintech, China) according to the manufacturer’s instructions.

Techniques: Isolation, Saline, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Enhanced therapeutic efficacy of repeated bone marrow–derived MSC administration in a murine model of pulmonary fibrosis

doi: 10.1186/s13287-025-04859-5

Figure Lengend Snippet: Limited retention and functional impairment of BMSCs following single intratracheal administration. A Levels of TNF-α, IL-6, and CXCL2 in mouse BALF measured by ELISA. Data represent four time-points after silicosis induction: D0, D7, D28, and D56. All data are presented as mean ± SD, n = 5 per group. *P < 0.05, **P < 0.01, *P < 0.001; two-way ANOVA. B Schematic overview of the experimental workflow. n = 3 per group. C , D Top 10 upregulated GO BP terms and KEGG pathways in BMSCs co-cultured with silicosis mouse BALF. E , F Top 10 downregulated GO BP terms and KEGG pathways in BMSCs co-cultured with silicosis mouse BALF

Article Snippet: ELISA assays were performed using commercial Mouse TNF-α, IL-6, and CXCL2 ELISA kits (Proteintech, China) according to the manufacturer’s instructions.

Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Stem Cell Research & Therapy

Article Title: Enhanced therapeutic efficacy of repeated bone marrow–derived MSC administration in a murine model of pulmonary fibrosis

doi: 10.1186/s13287-025-04859-5

Figure Lengend Snippet: Limited retention and functional impairment of BMSCs following single intratracheal administration. A Levels of TNF-α, IL-6, and CXCL2 in mouse BALF measured by ELISA. Data represent four time-points after silicosis induction: D0, D7, D28, and D56. All data are presented as mean ± SD, n = 5 per group. *P < 0.05, **P < 0.01, *P < 0.001; two-way ANOVA. B Schematic overview of the experimental workflow. n = 3 per group. C , D Top 10 upregulated GO BP terms and KEGG pathways in BMSCs co-cultured with silicosis mouse BALF. E , F Top 10 downregulated GO BP terms and KEGG pathways in BMSCs co-cultured with silicosis mouse BALF

Article Snippet: ELISA assays were performed using commercial Mouse TNF-α, IL-6, and CXCL2 ELISA kits (Proteintech, China) according to the manufacturer’s instructions.

Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Cell Culture